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Treatment with elicitors was carried out by Messiaen and Van Cutsem ; Chandra et al. All experiments were terminated by discharging the re- maining aequorin pool with 0. The output of the discriminator was captured by a Thorn-EMI photon- counting board and stored in an IBM-compatible computer for further analyses. For experiments performed in the presence of inhibitors dis- solved in dimethyl sulfoxide DMSO , control cells were treated with the same percentage 0. Extracellular H2O2 accumulation is transients induced by the two fractions of differently normalized per g cell fresh weight.
These and the following traces sized OGs are summarized in Table 1. Furthermore, in neither case was Table 1. OGs Bellincampi et al.
H2O2 accu- All these oligomers were able to induce a detectable mulation displayed the same dose-dependence Fig. Short OGs in fact have been shown to be respective concentrations.
An Fig. Cells were challenged with dif- ferent doses of OGs with either a DP of 9—18 a, b or 1—5 c, d. H2O2 concentration is normal- ized per g cell fresh weight Fig.
Soybean cells were challenged arrow with size-homogeneous OGs within the DP ranges 9—18 a and 1—5 b , tested at the same molar concentration 9 lM. OG mixtures with DP 9—18 a and 1—5 b were used as references. Inhibitor sensitivity of [Ca ]cyt elevations and H2O2 accumulation in soybean cells treated with OGs.
Control cells black trace of the main graph in a and c; filled diamonds in the insert were treated only with OGs, as described in Materials and methods. Extracellular H2O2 accumulation is normalized per g cell fresh weight b-glucan elicitor to their respective receptors in plant transient in our experimental system.
When cells, im- cells Stratmann et al. Neomycin sulphate, an cells retained the ability to respond to other stimuli such inhibitor of phospholipase C Legendre et al. The cell refractory state induced by OGs is reversed by the protein kinase inhibitor Ro and prolonged by the protein phosphatase inhibitor okadaic acid A refractory state, i.
Soybean ness of cells towards a second dose of OGs was found to cells were incubated with the following reagents: EGTA lM , increase even more Fig. For assays performed with EGTA, cells were previously washed extensively and resus- protein kinase inhibitors staurosporine and Ro On the other hand, Fig.
Soybean cells previously treated with OGs data not shown. The response to the second treatment is shown Discussion the G-protein-coupled receptor kinase inhibitor Ro In this paper we provide evidence for the involvement of Moore et al.
The DP of the OGs determined the relevant dif- inhibitor okadaic acid. The inhibitory effect of okadaic acid on response or H2O2 accumulation. Effect of the phosphatase inhibitor okadaic acid on the Fig. Effect of the protein kinase inhibitor Ro on the refractory state of soybean cells induced by OGs. Black trace, refractory state of soybean cells induced by OGs. Arrows indicate the transient induced by the first OG stimulus.
Furthermore, the concentrations of membrane, which go beyond the extracellular interfer- OGs required to induce morphogenetic effects doses ence with the binding of oligosaccharides to their to fold lower than those able to trigger defence receptor Stratmann et al.
The intriguing observation of a and no detectable extracellular H2O2 accumulation. Although TBB is generally ferent cellular responses.
What is still not known is believed to be a powerful and selective inhibitor of whether alternative signalling pathways are activated by protein kinase CK2 Espunya et al. The Several properties, are likely to be active participants in OG- elicitors are well known inducers of cell desensitization.
Nevertheless, the lack of Both microbial Binet et al.
The in cells repeatedly subjected to the same elicitor. Aequorin marker of the refractory state of cells. Two-piece composite Fuel Tank: Ready to fly in hours Warranty: This warranty does not cover any component parts damaged by use or modification. In no case shall Top Flite's liability exceed the original cost of the downloadd kit. Top Flite Cessna Skylane Gold Edition Airplane with decals, all hardware, clear plastic windshield and side glass, fiberglass tail cone, engine mount, cowl, three wheels, instruction manual and wheel pants.
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